SHM and Mammalian Cell Display

Coupling mammalian cell surface display with somatic hypermutation for the discovery and maturation of human antibodies

Bowers et al. Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20455-60. doi:10.1073/pnas.1114010108

Simultaneous surface display and secretion of proteins from Mammalian cells facilitate efficient in vitro selection and maturation of antibodies

Horlick et al. J Biol Chem. 2013 Jul 5;288(27):19861-9. doi:10.1074/jbc.M113.452482

Mammalian cell display for the discovery and optimization of antibody therapeutics

Bowers et al. Methods. 2014 Jan 1;65(1):44-56. doi:10.1016/j.ymeth.2013.06.010

Nucleotide insertions and deletions complement point mutations to massively expand the diversity created by somatic hypermutation of antibodies

Bowers et al. 2014 Nov 28;289(48):33557-67. doi:10.1074/jbc.M114.607176